Title:
養子細胞療法のための細胞を培養する方法
Document Type and Number:
Japanese Patent JP2013512694
Kind Code:
A
Abstract:
A method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cm 2 if the growth surface is not comprised of gas permeable material and up to 2 ml/cm 2 if the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5xl0 6 cells/cm 2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25 xl0 5 cells/cm 2 .
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Inventors:
Bella, Juan Ehu.
Rooney, Clio Em.
Lean, Anne M.
Wilson, John Earl.
Rooney, Clio Em.
Lean, Anne M.
Wilson, John Earl.
Application Number:
JP2012543271A
Publication Date:
April 18, 2013
Filing Date:
December 08, 2010
Export Citation:
Assignee:
WILSON WOLF MANUFACTURING CORPORATION
International Classes:
C12N1/00
Domestic Patent References:
| JPH10290689A | 1998-11-04 | |||
| JP2007061020A | 2007-03-15 | |||
| JPH11187868A | 1999-07-13 |
Foreign References:
| WO2008023786A1 | 2008-02-28 | |||
| WO2005051927A1 | 2005-06-09 |
Attorney, Agent or Firm:
Hironobu Onda
Makoto Onda
Atsushi Honda
Makoto Onda
Atsushi Honda