Title:
酵素リロケーションにより代謝経路のフラックスを制御する方法
Document Type and Number:
Japanese Patent JP2013526277
Kind Code:
A
Abstract:
Genetically manipulated cells, lysates of such cells, systems, and methods of use thereof are provided, where one or more enzymes in a pathway of interest are genetically modified to incorporate a peptide sequence that provides for relocation of the protein, e.g., to the periplasm, so as to sequester the enzyme, and where the enzyme controls flux in the pathway of interest.
Inventors:
Schwartz, James, Earl.
Application Number:
JP2013510190A
Publication Date:
June 24, 2013
Filing Date:
May 06, 2011
Export Citation:
Assignee:
GREENLIGHT BIOSCIENCES,INC.
International Classes:
C12N1/21; C12N1/15; C12N1/19; C12N5/10; C12N15/09
Domestic Patent References:
| JPWO2000039288A1 | ||||
| JP2009531050A | 2009-09-03 | |||
| JP2007534338A | 2007-11-29 |
Foreign References:
| WO2008094546A2 | 2008-08-07 | |||
| WO2005030949A1 | 2005-04-07 |
Other References:
J. AGRIC. CHEM., 2009, VOL. 57, PP. 8901-8905, JPN6015027576, ISSN: 0003111511
BIOTECHNOL. PROG., 2006, VOL. 22, PP. 406-410, JPN6015027577, ISSN: 0003111512
METHODS ENZYMOL., 2004, VOL. 388, PP. 145-156, JPN6015027578, ISSN: 0003111513
BIOCHEM BIOPHYS RES COMMUN., 1961年, 第5巻, 320-323ページ, JPN6014020448, ISSN: 0003327202
J AM CHEM SOC., 1955年, 第77巻, 4943-4944ページ, JPN6014020450, ISSN: 0003327203
MOL. BIOTECHNOL., 1999, VOL. 12, PP. 25-34, JPN6016020272, ISSN: 0003327201
J. BACTERIOL., 1990, VOL. 172, NO. 6, PP. 3358-3366, JPN6015027579, ISSN: 0003111514
APPL. MICROBIOL. BIOTECHNOL., 2009, VOL. 83, PP. 679-687, JPN6015027580, ISSN: 0003111515
BIOTECHNOL. PROG., 2006, VOL. 22, PP. 406-410, JPN6015027577, ISSN: 0003111512
METHODS ENZYMOL., 2004, VOL. 388, PP. 145-156, JPN6015027578, ISSN: 0003111513
BIOCHEM BIOPHYS RES COMMUN., 1961年, 第5巻, 320-323ページ, JPN6014020448, ISSN: 0003327202
J AM CHEM SOC., 1955年, 第77巻, 4943-4944ページ, JPN6014020450, ISSN: 0003327203
MOL. BIOTECHNOL., 1999, VOL. 12, PP. 25-34, JPN6016020272, ISSN: 0003327201
J. BACTERIOL., 1990, VOL. 172, NO. 6, PP. 3358-3366, JPN6015027579, ISSN: 0003111514
APPL. MICROBIOL. BIOTECHNOL., 2009, VOL. 83, PP. 679-687, JPN6015027580, ISSN: 0003111515
Attorney, Agent or Firm:
Kiyoji Kuzuwa