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Title:
方法および装置
Document Type and Number:
Japanese Patent JP2013528393
Kind Code:
A
Abstract:
A method for amplifying nucleic acid from a higher eukaryotic, such as mammalian or plant, nucleic acid source, the method comprising (a) contacting a sampling device with the source of higher eukaryotic, such as mammalian or plant, nucleic acid such that following said contacting, higher eukaryotic such as mammalian or plant nucleic acid-containing material is adhered to at least part of the sampling device, wherein the sampling device, or part thereof to which the nucleic acid-containing material is adhered, is made of a suitable polymeric material; (b) introducing the sampling device or part thereof to which the nucleic acid-containing material is adhered into a reaction vessel which contains a reaction mixture for carrying out a nucleic acid amplification reaction, without any prior treatment of the nucleic acid-containing material; and (c) performing a nucleic acid amplification reaction.

Inventors:
Paul Gerald Devenham
David John Moore
Application Number:
JP2013514785A
Publication Date:
July 11, 2013
Filing Date:
June 17, 2011
Export Citation:
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Assignee:
LGC Limited
International Classes:
C12N15/09; C12M1/00; C12M1/26; C12Q1/68
Domestic Patent References:
JP2005110687A2005-04-28
JP2005110687A2005-04-28
Foreign References:
WO2005032377A12005-04-14
US6103192A2000-08-15
WO1999034214A11999-07-08
WO2008030820A22008-03-13
WO2005032377A12005-04-14
US6103192A2000-08-15
WO1999034214A11999-07-08
WO2008030820A22008-03-13
Other References:
JPN5013010918; 'INSTRUCTIONS SHEET OF PHUSION HUMAN SPECIMEN DIRECT PCR KIT (THERMO SCIENTIFIC)' [ONLINE] , 20100614
JPN5013010919; 'DATA OF PUBLICATION OF XP055012357 (PHUSION HUMAN SPECIMEN DIRECT PCR KIT)' [ONLINE] , 20100614
JPN5013010920; FANG-CHIN WU: 'GENETIC POLYMORPHISMS OF 17 Y-CHROMOSOMAL SHORT TANDEM REPEAT LOCI IN ATAYAL POPULATION OF TAIWAN' CROATIAN MEDICAL JOURNAL V50 N3, 20090601, P313-320
JPN5013010918; 'INSTRUCTIONS SHEET OF PHUSION HUMAN SPECIMEN DIRECT PCR KIT (THERMO SCIENTIFIC)' [ONLINE] , 20100614
JPN5013010919; 'DATA OF PUBLICATION OF XP055012357 (PHUSION HUMAN SPECIMEN DIRECT PCR KIT)' [ONLINE] , 20100614
JPN5013010920; FANG-CHIN WU: 'GENETIC POLYMORPHISMS OF 17 Y-CHROMOSOMAL SHORT TANDEM REPEAT LOCI IN ATAYAL POPULATION OF TAIWAN' CROATIAN MEDICAL JOURNAL V50 N3, 20090601, P313-320
Attorney, Agent or Firm:
Yasuhiko Murayama
Masatake Shiga
Takashi Watanabe
Shinya Mitsuhiro