Title:
魚油から高純度のEPAを生成するためのSMB方法
Document Type and Number:
Japanese Patent JP2014518313
Kind Code:
A
Abstract:
The present invention provides a chromatographic separation process for recovering a polyunsaturated fatty acid (PUFA) product from a feed mixture which is a fish oil or which is derived from fish oil, which process comprises the steps of:
(i) purifying the feed mixture in a chromatographic separation step, to obtain a first intermediate product; and
(ii) purifying the first intermediate product obtained in (i) in a simulated or actual moving bed chromatographic separation step, to obtain a second intermediate product; and
(iii) purifying the second intermediate product obtained in (ii) in a chromatographic separation step using a different chromatographic apparatus to that used in the second separation step, to obtain the PUFA product; wherein
an aqueous organic solvent is used as eluent in each separation step;
saturated and/or monounsaturated fatty acids present in the feed mixture are removed in the first separation step;
the PUFA product is separated from different components of the feed mixture in steps (ii) and (iii); and
the PUFA product obtained in the third separation step contains EPA or an EPA derivative in an amount greater than 90 wt%.
Inventors:
Keriher, Adam
Morrison, Angus
Orroscar, Anil
Nile Rema, Lachesh Viculaman
Agawar, Abhilesh
Morrison, Angus
Orroscar, Anil
Nile Rema, Lachesh Viculaman
Agawar, Abhilesh
Application Number:
JP2014517955A
Publication Date:
July 28, 2014
Filing Date:
July 06, 2012
Export Citation:
Assignee:
BIS F Pharma (Cornish) Limited
International Classes:
C11B3/16; C11B1/10
Domestic Patent References:
| JPH08512336A | 1996-12-24 | |||
| JP2013516398A | 2013-05-13 |
Foreign References:
| WO2001087452A2 | 2001-11-22 |
Other References:
YI XIE, ET AL.: "Standing wave design and experimental validation of a tandem simulated moving bed process for insuli", BIOTECHNOLOGY PROGRESS, vol. 18, no. 6, JPN6014041508, 2002, pages 1332 - 1344, XP001148482, ISSN: 0003308923, DOI: 10.1021/bp025547r
Attorney, Agent or Firm:
Hiroshi Kobayashi
Norio Omori
Yasuhito Suzuki
Norio Omori
Yasuhito Suzuki