Title:
メバロン酸、イソプレン及びイソプレノイドの生産量を増加させるための組み換え微生物
Document Type and Number:
Japanese Patent JP2014519812
Kind Code:
A
Abstract:
The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus).
Inventors:
Beck, the Zucker Lee cue
A mirror, Michael See
Pelles, Caroline M
A プライ mark, ユリヤ Ey
Pucci, Jeff Py
Verres, Derek h
A mirror, Michael See
Pelles, Caroline M
A プライ mark, ユリヤ Ey
Pucci, Jeff Py
Verres, Derek h
Application Number:
JP2014508164A
Publication Date:
August 21, 2014
Filing Date:
April 27, 2012
Export Citation:
Assignee:
ダニスコ you S ink
The Goodyear Tire & Rubber company
The Goodyear Tire & Rubber company
International Classes:
C12N1/21; C12N1/15; C12N1/19; C12N15/09; C12P5/00; C12P7/42
Domestic Patent References:
| JP2011505841A | 2011-03-03 | |||
| JP2009538601A | 2009-11-12 | |||
| JP5706444B2 | 2015-04-22 |
Foreign References:
| WO2009143490A1 | 2009-11-26 |
Other References:
BIOTECHNOLOGY LETTERS, vol. 26, JPN6016010290, 2004, pages 1487 - 1491, ISSN: 0003279359
SAVADZE, G. A. ET AL.: "Changes in the specific activity of carbon-14 dioxide and isoprene at the compensation point", CAPLUS[ONLINE], JPN6016010288, 1977, ISSN: 0003279360
SAVADZE, G. A. ET AL.: "Changes in the specific activity of carbon-14 dioxide and isoprene at the compensation point", CAPLUS[ONLINE], JPN6016010288, 1977, ISSN: 0003279360
Attorney, Agent or Firm:
Yukuzo Yamazaki
Toshiaki Akamatsu
尾首 亘聰
Masako Okutani
Tadao Naito
Imai 千裕
Yoshiko Takeyama
Toshiaki Akamatsu
尾首 亘聰
Masako Okutani
Tadao Naito
Imai 千裕
Yoshiko Takeyama