Title:
MEASUREMENT OF INTACT MASS OF PROTEIN-CONJUGATED AGENT COMPOUNDS
Document Type and Number:
Japanese Patent JP2018063265
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide methods and systems for rapid measurement of the intact mass of non-covalently bonded antibody heavy chains (HC) and light chains (LC) which result from bonding of drug conjugates to interchain cysteine residues.SOLUTION: By analyzing an antibody-drug conjugate (ADC) under native desalting conditions, an intact-bivalent structure of the ADC, which ordinarily may decompose as a consequence of denaturing chromatographic conditions typically used for LCMS, is maintained. The mass of the desalted ADC is subsequently determined using desolvation and ionization ESI-MS conditions. The methods described herein provide direct measurement of the intact mass of an ADC conjugated at interchain cysteine residues. The methods described herein also provide the relative quantitation of individual ADC species.SELECTED DRAWING: Figure 1
Inventors:
JOHN FAY VALLIERE-DOUGLASS
OSCAR SALAS
OSCAR SALAS
Application Number:
JP2018002748A
Publication Date:
April 19, 2018
Filing Date:
January 11, 2018
Export Citation:
Assignee:
SEATTLE GENETICS INC
International Classes:
G01N27/62; A61K39/395; G01N30/72; G01N30/88; G01N33/68
Domestic Patent References:
| JP2007532882A | 2007-11-15 | |||
| JP2005524086A | 2005-08-11 |
Foreign References:
| US20090325311A1 | 2009-12-31 | |||
| US20050176062A1 | 2005-08-11 |
Attorney, Agent or Firm:
Atsushi Aoki
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Ryotaro Takei
Hidena Tsuda
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Ryotaro Takei
Hidena Tsuda