Title:
CELL CULTURE SYSTEM AND CELL CULTURE METHOD
Document Type and Number:
Japanese Patent JP2019213497
Kind Code:
A
Abstract:
To provide a cell culture system and a cell culture method capable of efficiently collecting useful substances such as antibody from a liquid medium.SOLUTION: A cell culture system comprises: a culture tank for storing a liquid medium contained cells to be cultured; a fluid force separation apparatus for separating the liquid medium supplied from the culture tank into a rich cell fraction with a relatively high cell density and a lean cell fraction with a relatively low cell density; and a filtration separation apparatus for removing cells from the lean cell fraction separated by the fluid force separation apparatus to collect the liquid medium. The fluid force separation apparatus separates the liquid medium using a vortex flow which is generated by flowing on a curved channel having a rectangular cross section. Alternatively, the lean cell fraction is supplied to the culture tank from the fluid force separation apparatus to reduce the cell density of the liquid medium of the culture tank, and then the liquid medium is supplied to the filtration separation apparatus to remove cells and collect the liquid medium.SELECTED DRAWING: Figure 1
Inventors:
IKEDA RYOSUKE
ISO YOSHIYUKI
KAMEKURA KOICHI
MIZUNUMA MASATO
ISO YOSHIYUKI
KAMEKURA KOICHI
MIZUNUMA MASATO
Application Number:
JP2018112890A
Publication Date:
December 19, 2019
Filing Date:
June 13, 2018
Export Citation:
Assignee:
IHI CORP
IHI PLANT ENG CORP
IHI PLANT ENG CORP
International Classes:
C12M1/00; C12N1/00
Domestic Patent References:
| JP2017527299A | 2017-09-21 |
Foreign References:
| WO2018017022A1 | 2018-01-25 | |||
| WO2013109520A1 | 2013-07-25 |
Attorney, Agent or Firm:
Hidekazu Miyoshi
Shunichi Takahashi
Masakazu Ito
Toshio Takamatsu
Shunichi Takahashi
Masakazu Ito
Toshio Takamatsu