Title:
【発明の名称】豚のY―精子の検定方法
Document Type and Number:
Japanese Patent JP2511445
Kind Code:
B2
Abstract:
PURPOSE:To permit assay of Y-spermatozoa by previously cleaning the spermatozoa of a mammal with a phosphate buffer soln. and treating the same with protease, dyeing the Y-spermatozoa (the spermatozoa having Y chromosomes) of such spermatozoa with quinacrine mustard to that the F-bodies (small bodies which emit fluorescence) can be visually observed. CONSTITUTION:The Y-spermatozoa of the spermatozoa of the mammal which are previously cleaned by the phosphate buffer soln. such as Ringer's soln. and are treated by the protease such as 'Dispase(R)' are dyed by the quinacrine mustard. The dyeing by the quinacrine mastered is executed by using a quinacrine mustard soln. prepd. by dissolving the quinacrine mustard at about 0.0025-0.0050% in the phosphate buffer soln. The dyed F-bodies can be observed by inspecting the dyed spermatozoa with a fluorescent microscope. The number of the Y-spermatozoa is known by counting the number of the dyed F-bodies.
Inventors:
OGAWA SHOZO
NAGASHIMA HIROSHI
NAGASHIMA HIROSHI
Application Number:
JP5035087A
Publication Date:
June 26, 1996
Filing Date:
March 06, 1987
Export Citation:
Assignee:
NISSHIN FLOUR MILLING CO
International Classes:
A61D19/00; A61B17/425; G01N1/30; G01N33/48; G01N33/50; A61B17/43; (IPC1-7): G01N33/48
Domestic Patent References:
| JP5356381A | ||||
| JP60114137A |
Other References:
Ｎａｔｕｒｅ，２２６，（１９７０），Ｐ．９６１−９６２
丹羽太左衛門、「最新・家畜の人工受精」、初版（昭和４５年３月１５日）、明文書房，Ｐ．２６６−２７１
丹羽太左衛門、「最新・家畜の人工受精」、初版（昭和４５年３月１５日）、明文書房，Ｐ．２６６−２７１
Attorney, Agent or Firm:
Chika Takagi (2 outside)