Title:
【発明の名称】ポリヌクレオチド合成用ホスホルアミダイト
Document Type and Number:
Japanese Patent JP2801564
Kind Code:
B2
Abstract:
In a method for prepg. oligomers of monomers which include members having common functionalities for condensation, but otherwise differing as to compsn., in which (1) the oligomers are prepd. by sequential addn. of terminally blocked monomers, the monomers having one or more reactive functionalities joined to protective gps., while the growing chain is bound to a support, (2) terminal blocking gps. are removed and the next monomer added and (3) after the final monomer addn., the terminal blocking gp. is cleaved from the support, the improvement comprises (a) prior to each sequential addn., capping terminal gps. which are not blocked with a selectively removable capping gp., (b) after the last monomer addn., removing capping gps. and any enzymatic hydrolysis interfering protecting gps. and (c) enzymatically hydrolysing oligomers lacking a terminal blocking gp., prior to removal of terminal blocking gps.
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Inventors:
URDEA MICHAEL S (US)
HORN THOMAS (US)
HORN THOMAS (US)
Application Number:
JP20082895A
Publication Date:
September 21, 1998
Filing Date:
August 07, 1995
Export Citation:
Assignee:
CHIRON CORP (US)
International Classes:
C07F9/09; C07F9/24; C07F9/6561; C07H19/06; C07H19/16; C07H21/00; C12N15/09; C07H21/04; C07K1/06; C07K1/113; C07K1/14; C12N15/00; C12P19/34; C12P21/00; (IPC1-7): C07F9/24; C07H19/06; C07H19/16; C07K1/06; C12N15/09
Attorney, Agent or Firm:
Takashi Ishida (4 others)