Title:
【発明の名称】7-(4-カルボキシブタンアミド)-セファロスポラン酸アシラーゼの製法
Document Type and Number:
Japanese Patent JP2805594
Kind Code:
B2
Abstract:
The present invention relates to a process for producing large amounts of 7 beta -(4-carboxybutanamido)-cephalosporanic acid acylase ("GA") enzyme, which process consists in growing, under suitable culture conditions, a recombinant Escherichia coli K12 strain and subsequently extracting the enzyme from the resulting culture. Enzyme production is controlled by a particular expression system which can be induced by corn-steep liquor, an extremely low cost component of the culture medium. The enzyme can be used in the enzymatic preparation of 7-amino-cephalosporanic acid by starting from 7 beta -(4-carboxybutanamido)-cephalosporanic acid.
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Inventors:
Wilhelms van der Goes
Antonella Bernardi
Aldo Bozzec
Juliana Francozi
Pietro Cesti
Antonella Bernardi
Aldo Bozzec
Juliana Francozi
Pietro Cesti
Application Number:
JP1986795A
Publication Date:
September 30, 1998
Filing Date:
January 13, 1995
Export Citation:
Assignee:
Ministero de Luni Bersita e de la Richelca Scientifica e Techca
International Classes:
C12N1/21; C12N9/80; C12N9/86; C12N15/00; C12N15/31; C12N15/55; C12N15/70; C12P35/02; C12N15/09; C12R1/19; C12R1/38; (IPC1-7): C12N9/86; C12N1/21; C12N15/09
Other References:
【文献】欧州特許出願公開469919(EP,A)
Attorney, Agent or Firm:
Asakura Shozo