Title:
【発明の名称】β-ラクタム抗生物質の酵素による測定方法
Document Type and Number:
Japanese Patent JP2823382
Kind Code:
B2
Abstract:
It entails the following stages: 1) incubation of the biological fluid with the soluble D-alanyl-D-alanine carboxypeptidase produced by the strain Actinomadura R39; 2) incubation of the mixture obtained with a solution of substrate, which is a thioester of formula
in the presence of an amino acid of D configuration or glycine; 3) determination of the quantity of 2-mercaptoalkanoic acid of formula
formed in stage 2); 4) comparison of the determination of stage 3) with a standard.
In these formulae:
R1 = benzoyl, phenylacetyl or Nalpha-acetyl-Llysyl,
R2 = glycyl or D-alanyl, and
R3 = H or CH3.
The invention also relates to a test kit comprising the reagents to be employed for implementation of the method.
Inventors:
Jack degueraen
Jean-Marie Frere
Jean-Marie Frere
Application Number:
JP9676291A
Publication Date:
November 11, 1998
Filing Date:
April 26, 1991
Export Citation:
Assignee:
Yusebe-Bioproducts Society Anonym
International Classes:
C07K5/068; C07K14/505; C12Q1/37; A61K38/00; C12R1/03; (IPC1-7): C12Q1/37
Attorney, Agent or Firm:
Akira Asamura (3 outside)