Title:
【発明の名称】アルカリホスファターゼ及びその製造法
Document Type and Number:
Japanese Patent JP2876645
Kind Code:
B2
Abstract:
PURPOSE:To obtain the title phosphatase resistant to inhibition by inorganic phosphoric acid as a degradation product from enzymatic reaction, thus useful as a labelled enzyme for the enzyme immunoassay by using microorganisms short in culture period, classified as thermophilic Bacillus. CONSTITUTION:Microorganisms classified as thermophilic Bacillus and capable of producing alkali phosphatase resistant to inhibition by inorganic phosphoric acid [pref. Bacillus stearothermophilus TB-443 (FERM P-10522)] is put to culture, and said alkali phosphatase is produced and accumulated in the culture solution, and then collected, thus obtaining the objective phosphatase resistant to inhibition by inorganic phosphoric acid. For the culture conditions, it is suggested that the temperature be 45-55 deg.C and the time 6-24hr.
Inventors:
MAEHANE HIDEO
MITANI MASARU
TAKASHIO HITOCHIKA
MITANI MASARU
TAKASHIO HITOCHIKA
Application Number:
JP24175689A
Publication Date:
March 31, 1999
Filing Date:
September 20, 1989
Export Citation:
Assignee:
TOYO BOSEKI KK
International Classes:
C12N9/16; C12R1/07; (IPC1-7): C12N9/16