Title:
【発明の名称】接合転写/翻訳の無細胞系における遺伝子の予備発現法
Document Type and Number:
Japanese Patent JP3194928
Kind Code:
B2
Abstract:
Prodn. of polypeptides is effected by expression of genes in the form of DNA mols. in a cell-free transcription/translation system contg. amino acids, ATP, GTP, CTP and UTP as substrates, resulting in the formation of prods. including polypeptides, AMP, ADP, GDP, CDP, UDP, pyrophosphates and inorganic phosphates. The process is operated continuously, where the prods. are withdrawn as they are formed and the substrates are added so as to maintain their initial concns.
Inventors:
Baranov, Vladimir Ivanowich
Morozov, Igor Yuriewich
Spirin, alexander sergeie witch
Morozov, Igor Yuriewich
Spirin, alexander sergeie witch
Application Number:
JP50222989A
Publication Date:
August 06, 2001
Filing Date:
January 27, 1989
Export Citation:
Assignee:
Institute, Belka, Ross Iskoy, Academia, Nauk
International Classes:
C07K1/02; C12N9/00; C12N9/06; C12N15/09; C12N9/86; C12P19/34; C12P21/00; C12P21/02; (IPC1-7): C12N9/00; C12N15/09; C12P21/00
Other References:
Ｅｕｒ．Ｊ．Ｂｉｏｃｈｅｍ．，１６０（１）（１９８６），ｐ．６９−７５
Ｇｅｎｅ，８４（１９８９），ｐ．４６３−４６６
Ｓｃｉｅｎｃｅ，２４２（４８８２）（１９８８），ｐ．１１６２−６４
Ｇｅｎｅ，８４（１９８９），ｐ．４６３−４６６
Ｓｃｉｅｎｃｅ，２４２（４８８２）（１９８８），ｐ．１１６２−６４
Attorney, Agent or Firm:
Kazuo Sato (2 outside)