【発明の名称】カルボキシメチルアミロースに結合させた結合メンバーを使用するイオン捕獲アッセイ

Title:

【発明の名称】カルボキシメチルアミロースに結合させた結合メンバーを使用するイオン捕獲アッセイ

Document Type and Number:

Japanese Patent JP3267614

Kind Code:

B2

Abstract:

The present invention includes novel assays employing a capture reagent, involving a first binding member conjugated to a polymeric anion such as carboxymethylamylose, and a solid phase material containing a reaction site comprising a polymeric cation substance. A test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. The use of carboxymethylamylose to prepare a suitably charged capture reagent provides a superior capture reagent that is capable of binding and retaining the analyte on the solid phase even in the presence of polyanionic non-specific binding blockers.

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Inventors:

Adam Twick, Jeanina
Berry, Daniel S
Jyou, E-ha
Stroup, Stephen Denham

Application Number:

JP50039693A

Publication Date:

March 18, 2002

Filing Date:

April 10, 1992

Export Citation:

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Assignee:

Abbot Laboratories

International Classes:

G01N33/531; G01N33/538; G01N33/543; G01N33/547; G01N33/548; (IPC1-7): G01N33/543; G01N33/531; G01N33/547

Domestic Patent References:

JP344399A
JP2168162A
JP60500548A

Other References:

【文献】米国特許4121975(US,A)

Attorney, Agent or Firm:

Yoshio Kawaguchi (2 outside)

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