Title:
【発明の名称】高効率抗体スクリーニング法
Document Type and Number:
Japanese Patent JP3375941
Kind Code:
B2
Abstract:
The present invention provides a method in which antibodies against target proteins contained in samples are screened with high efficiency. Samples including protein groups are treated with two-dimensional electrophoresis, the aforementioned protein groups are developed on a two-dimensional electrophoretic gel as an individual spot protein, the spot protein that had been developed is visualized after being transcribed onto a solid phase, then, after isolating each of the spot proteins, it is reacted with a phage antibody library, antibody that bonded to the spot proteins are replicated, an operation wherein said replicated antibody is reacted with the aforementioned spot protein is conducted once or more than twice, and antibodies against specific proteins contained in the samples including protein groups are screened. As samples including protein groups, it is preferable to use a samples wherein cellular subfraction had been separated and purified beforehand and samples wherein posttranslational modification had been removed.
Inventors:
Kiyotoshi Kaneko
Application Number:
JP2000358539A
Publication Date:
February 10, 2003
Filing Date:
November 24, 2000
Export Citation:
Assignee:
Japan Science and Technology Agency
President, National Center of Neurology and Psychiatry
President, National Center of Neurology and Psychiatry
International Classes:
G01N33/53; C07K16/00; C07K16/44; C40B30/04; G01N33/15; G01N33/50; G01N33/68; G01N37/00; (IPC1-7): G01N33/53; G01N33/15; G01N33/50; G01N37/00
Domestic Patent References:
| JP6160384A | ||||
| JP62267297A |
Other References:
【文献】国際公開00/9464(WO,A1)
【文献】国際公開98/59361(WO,A1)
【文献】国際公開98/59361(WO,A1)
Attorney, Agent or Firm:
Masaki Hirota