Title:
【発明の名称】フィブリノーゲン、XIII因子及びフィブロネクチンを含んで成る生物学的接着剤の獲得方法
Document Type and Number:
Japanese Patent JP3492694
Kind Code:
B2
Abstract:
The present invention relates to a process of making a concentrate of coagulation proteins starting with whole human or animal plasma. This concentrate is used as a biological adhesive when extemporaneously mixed to thrombin. The concentrated proteins include mostly fibrinogen, fibrin stabilizing factor (factor XIII) and fibronectin. The claimed process has the advantage of being short of execution while providing an excellent yield of coagulable proteins. No protease inhibitor has to be added during the process. The process involves steps of separation by "salting-out" in presence of amino-6 hexanoic acid which prevents co-precipitation of plasminogen with the desired coagulable proteins. The proteins so obtained are very stable after reconstitution in water for at least 24 hours at room or body temperature. After mixing with thrombin and calcium, the adhesive shows excellent strength and biocompatibility.
More Like This:
Inventors:
Buoy-cuck, trung
La Boire, Rize
Michael St Pico, Dominic
La Boire, Rize
Michael St Pico, Dominic
Application Number:
JP52203595A
Publication Date:
February 03, 2004
Filing Date:
February 28, 1994
Export Citation:
Assignee:
Haemakyua Biotech Inc.
International Classes:
A61L24/04; A61L24/10; A61L26/00; A61L24/00; C07K1/30; C07K1/34; C07K1/36; C07K14/75; C07K14/78; C12N9/10; A61K38/00; C07K; (IPC1-7): C07K14/75; A61L24/00; C07K1/30; C07K1/34; C07K1/36
Attorney, Agent or Firm:
Takashi Ishida (2 outside)