Title:
核酸分子を特性化する方法
Document Type and Number:
Japanese Patent JP3561523
Kind Code:
B2
Abstract:
A method of characterizing a nucleic acid molecule is disclosed. The method comprises synthesizing DNA in the presence of a reaction mixture comprising a nucleic acid template, a primer molecule, an enzyme that extends the primer so that a DNA molecule may be synthesized, four canonical deoxynucleoside triphosphates and at least one non-canonical deoxynucleoside triphosphate. The non-canonical deoxynucleoside triphosphate is incorporated into the synthesized DNA in place of a portion of only one canonical deoxynucleoside triphosphate. The synthesized DNA is treated with an N-glycosylase that excises a base portion of the non-canonical deoxynucleoside triphosphate from the synthesized DNA. The DNA is then treated in such a manner that the phosphodiester backbone of the DNA is broken at the abasic site, thus creating at least two DNA fragments. The fragments are separated according to size.
Inventors:
The End Lisa, The Jerome.
Hoffman, Leslie M.
Smith, Robert E.
Hoffman, Leslie M.
Smith, Robert E.
Application Number:
JP51359497A
Publication Date:
September 02, 2004
Filing Date:
September 25, 1996
Export Citation:
Assignee:
Epicenter Technologies Corporation
International Classes:
C12N15/00; C12N15/09; C12Q1/68; G01N33/53; (IPC1-7): C12N15/09; C12Q1/68; G01N33/53
Attorney, Agent or Firm:
Takao Kawasaki
Toshizo Iida
Toshizo Iida