Title:
多相系におけるイムノアッセイの実施方法
Document Type and Number:
Japanese Patent JP3580504
Kind Code:
B2
Abstract:
The present invention relates to a method of carrying out an immunoassay in a multiphase system. A sample containing an analyte is brought into contact with a receptor A and a tracer. The analyte can either form a complex with the tracer, or counteract the formation of a complex of receptor A and tracer by competing with the tracer for binding to receptor A, or counteract the formation of a complex of receptor A and tracer by competing with receptor A. Receptor B is added and the signal is determined. In this method, receptor A and receptor B are suitably immobilized, ensuring that the tracer either cannot enter into any binding involving the simultaneous participation of receptors A and B or can enter into such a binding to only such a slight extent that it is nevertheless possible to detect and differentiate differing analyte concentrations.
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Inventors:
Neuenhofer, Shyte Juan
Kesmarker, Reinhard
Kesmarker, Reinhard
Application Number:
JP50280096A
Publication Date:
October 27, 2004
Filing Date:
June 23, 1995
Export Citation:
Assignee:
Dade Behring Marburg Gezershyaft Mitt Besilenktel Haftung
International Classes:
G01N33/542; C12N9/96; C12Q1/68; G01N33/53; G01N33/532; G01N33/543; G01N33/566; G01N33/574; G01N33/577; G01N33/58; (IPC1-7): G01N33/542; C12Q1/68; G01N33/577
Domestic Patent References:
| JP55135753A | ||||
| JP63159759A |
Attorney, Agent or Firm:
Chika Takagi
Kosuke Nishimura
Kosuke Nishimura