Title:
SINE法による動物種判別方法
Document Type and Number:
Japanese Patent JP3600891
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a method for discriminating animal species by SINE(short interspersed element) method. SOLUTION: This method comprises the steps of preparing each genomic DNA library from at least one animal species to be discriminated, isolating each clone including an orthologous gene locus specifically inserted with a SINE family or subfamily in at least one of the animal species, amplifying by PCR the sequence of the gene locus for each of the animal species using a set of PCR primers annealing on the flanking sequences located on both ends of the SINE family or subfamily in the gene locus, and discriminating the animal species based on the presence/absence of a band indicating the presence of the gene locus by making a gel electrophoresis of the resultant PCR product.
Inventors:
Norihiro Okada
Application Number:
JP2001126667A
Publication Date:
December 15, 2004
Filing Date:
April 24, 2001
Export Citation:
Assignee:
Foundation for Science and Engineering
International Classes:
G01N33/50; C12N15/09; C12Q1/68; G01N33/00; G01N33/53; G01N33/566; G06F17/30; (IPC1-7): C12N15/09; C12Q1/68
Other References:
Nature,1997年,Vol.388,p.666-670
Physiol.Genomics,2000年,Vol.4,p.13-24
Genetics,2001年 2月,No.157,p.777-784
Proc.Natl.Acad.Sci.USA,1993年,Vol.90,p.6995-6999
Proc.Natl.Acad.Sci.USA,1994年,Vol.91,p.10153-10157
Genetics,1996年,No.142,p.915-926
Can.J.Fish.Aquat.Sci.,1998年,Vol.55,No.8,p.1864-1870
関税中央分析所報,2000年,No.39,p.35-39
Mol.Biol.Evol.,1999年,Vol.16,No.8,p.1046-1060
Mamm.Genome,1998年,Vol.9,No.7,p.550-555
Physiol.Genomics,2000年,Vol.4,p.13-24
Genetics,2001年 2月,No.157,p.777-784
Proc.Natl.Acad.Sci.USA,1993年,Vol.90,p.6995-6999
Proc.Natl.Acad.Sci.USA,1994年,Vol.91,p.10153-10157
Genetics,1996年,No.142,p.915-926
Can.J.Fish.Aquat.Sci.,1998年,Vol.55,No.8,p.1864-1870
関税中央分析所報,2000年,No.39,p.35-39
Mol.Biol.Evol.,1999年,Vol.16,No.8,p.1046-1060
Mamm.Genome,1998年,Vol.9,No.7,p.550-555
Attorney, Agent or Firm:
Takashi Ishida
Jun Tsuruta
Kazuhiro Nakamura
Masaya Nishiyama
Higuchi Souji
Jun Tsuruta
Kazuhiro Nakamura
Masaya Nishiyama
Higuchi Souji