Title:
DNAの合成方法
Document Type and Number:
Japanese Patent JP3748378
Kind Code:
B2
Abstract:
A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3' 5' exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3' 5' exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3' 5' exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase. According to the present invention, DNA synthesis can be carried out at an efficiency more excellent as compared to conventional DNA synthesis reaction.
Inventors:
Kiyozo Asada
Takashi Uemori
Yoshimi Sato
Tomoko Fujita
Kazue Miyake
Takeda Osamu
Hiroyuki Mukai
Ikunoshin Kato
Takashi Uemori
Yoshimi Sato
Tomoko Fujita
Kazue Miyake
Takeda Osamu
Hiroyuki Mukai
Ikunoshin Kato
Application Number:
JP2000544787A
Publication Date:
February 22, 2006
Filing Date:
April 21, 1999
Export Citation:
Assignee:
Takara Bio Inc.
International Classes:
C12N15/09; C12N9/12; C12N9/96; C12P19/34; C12Q1/68
Domestic Patent References:
| JP6277062A |
Other References:
Bio Techniques,1992,Vol.12,No.3,p.332-334
Proc.Natl.Acad.Sci.USA,1994,Vol.91,No.12,p.5695-5699
Bio Techmiques,1997,Vol.23,No.1,p.24-28
Methods Mol.Cel.Biol.,1995,Vol.5,No.2,p.122-124
Proc.Natl.Acad.Sci.USA,1994,Vol.91,No.12,p.5695-5699
Bio Techmiques,1997,Vol.23,No.1,p.24-28
Methods Mol.Cel.Biol.,1995,Vol.5,No.2,p.122-124
Attorney, Agent or Firm:
Yoshinori Hosoda