Title:
緑色蛍光タンパク質の使用
Document Type and Number:
Japanese Patent JP3810791
Kind Code:
B2
Abstract:
PCT No. PCT/US94/10165 Sec. 371 Date Jun. 18, 1996 Sec. 102(e) Date Jun. 18, 1996 PCT Filed Sep. 9, 1994 PCT Pub. No. WO95/07463 PCT Pub. Date Mar. 16, 1995This invention provides a cell comprising a DNA molecule having a regulatory element from a gene, other than a gene encoding a green fluorescent protein operatively linked to a DNA sequence encoding the green fluorescent protein. This invention also provides living organisms which comprise the above-described cell. This invention also provides a method for selecting cells expressing a protein of interest which comprises: a) introducing into the cells a DNAI molecule having DNA sequence encoding the protein of interest and DNAII molecule having DNA sequence encoding a green fluorescent protein; b) culturing the introduced cells under conditions permitting expression of the green fluorescent protein and the protein of interest; and c) selecting the cultured cells which express green fluorescent protein, thereby selecting cells expressing the protein of interest. Finally, this invention provides various uses of a green fluorescent protein.
Inventors:
Chalphy, martin
Prussia, Douglas
Prussia, Douglas
Application Number:
JP50880195A
Publication Date:
August 16, 2006
Filing Date:
September 09, 1994
Export Citation:
Assignee:
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
International Classes:
C12N15/09; G01N33/48; C07K14/435; C12N1/15; C12N1/21; C12N5/10; C12N15/65; C12P21/02; C12Q1/02; C12Q1/42; C12Q1/68; G01N33/18; G01N33/58
Domestic Patent References:
| JP5501862A |
Other References:
Gene, 1992年,111, 229-233
Science 263(5148) p.802-805 (1994)
Photochem Photobiol 62(4) p.651-656 (1995)
Science 263(5148) p.802-805 (1994)
Photochem Photobiol 62(4) p.651-656 (1995)
Attorney, Agent or Firm:
Takehiko Suzue
Sadao Muramatsu
Ryo Hashimoto
Sadao Muramatsu
Ryo Hashimoto