Title:
天疱瘡モノクローナル抗体
Document Type and Number:
Japanese Patent JP3817587
Kind Code:
B2
Abstract:
The present invention provides a monoclonal antibody having a pathogenic activity that can induce pemphigus lesion, a peptide specifically recognized by the monoclonal antibody and useful as a therapeutic drug for pemphigus autoimmune disease, etc. As anti-mouse Dsg3 antibody-producing cells are present in the splenocytes of pemphigus vulgaris mouse model constructed by using autoantigen knockout mouse, cell fusion was conducted with the splenocytes of said mouse model and mouse myeloma cells using polyethyleneglycol, hybridomas were constructed, and monoclonal antibodies against Dsg3 were constructed. Among them, a monoclonal antibody having a pathological activity that can induce pemphigus lesions was screened, the base sequence and amino acid sequence in its variable region (heavy chain, light chain) was determined, and the specific epitope part was identified.
Inventors:
Kazuyuki Tsunoda
Masayuki Amaya
Takeji Nishikawa
Shigeo Oyasu
Masayuki Amaya
Takeji Nishikawa
Shigeo Oyasu
Application Number:
JP2003525039A
Publication Date:
September 06, 2006
Filing Date:
September 04, 2002
Export Citation:
Assignee:
Keio University
International Classes:
C07K16/18; A01K67/027; A61K38/17; A61P31/12; A61P37/02; A61P43/00; C07K14/00; C07K16/28; C12N5/20; C12N15/02; C12N15/13; C12P21/08; G01N33/15; G01N33/48; G01N33/50; G01N33/53; G01N33/564; A61K38/00; A61P17/00; A61P37/06
Domestic Patent References:
| JP8188540A |
Other References:
J Invest Dermatol,Aug.,2001,Vol.117,No.2,p.394,028
J Invest Dermatol,2000,Vol.114,No.4,p.846,579
J Invest Dermatol,July,2002,Vol.119,No.1,p.228,125
J Invest Dermatol,2000,Vol.114,No.4,p.846,579
J Invest Dermatol,July,2002,Vol.119,No.1,p.228,125
Attorney, Agent or Firm:
Masaki Hirota