Title:
プロテアーゼの自己触媒的に活性化可能なチモーゲン前駆体及びそれらの使用
Document Type and Number:
Japanese Patent JP3947653
Kind Code:
B2
Abstract:
A process for the recombinant production of a protease is characterised by (a) transformation of a host cell with a recombinant nucleic acid which codes for a zymogenic protease precursor containing an autocatalytic cleavage site which does not occur naturally, this cleavage site being recognised by the active form of said protease, cleaving the zymogenic precursor into the active protease; (b) cultivation of the host cell such that the zymogenic protease precursor is created in the host cell in the form of inclusion bodies; (c) isolation and naturalisation of the inclusion bodies in such conditions that the protease part of the zymogenic precursor is produced with its natural conformation; and (d) autocatalytic cleavage of the naturalised zymogenic precursor to produce the active protease. This process is suitable to produce recombinant proteases in a simple manner and with high yields.
Inventors:
Kopetski, Erhard
Hopfner, Karl-Peter
Board, wolfram
Hoover, Robert
Hopfner, Karl-Peter
Board, wolfram
Hoover, Robert
Application Number:
JP2000507811A
Publication Date:
July 25, 2007
Filing Date:
August 12, 1998
Export Citation:
Assignee:
Roche Diagnostics Gaem Behr
International Classes:
C12N15/09; C07K14/745; C12N9/50; C12N9/64; C12N9/76; C12N15/57; C12P21/06
Other References:
J. Biol. Chem., Vol. 263, No. 26, p. 12959-12963,1988年
Gene, Vol. 41, p. 305-310,1986年
Biochim. Biophys Acta., Vol. 839, No. 3, p. 262-267,1985年
Gene, Vol. 41, p. 305-310,1986年
Biochim. Biophys Acta., Vol. 839, No. 3, p. 262-267,1985年
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Ishii Teiji
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