Title:
核酸分析用プローブおよび検出方法
Document Type and Number:
Japanese Patent JP3992079
Kind Code:
B2
Abstract:
A probe for detecting nonradioactive nucleic acids by the hybridization technique, and a highly sensitive and highly selective method for the detection. The probe comprises a set of two or more oligonucleotide probes that can hybridize perfectly complementarily with the specific portion of the sequence of the consecutive bases of the target nucleic acid, each oligonucleotide probe being labeled at the 5' or 3' end thereof with a chromophoric group having a suitable spatial arrangement so that adjacent chromophoric groups can yield an excimer or the like when each probe hybridizes with the target nucleic acid. More specifically, although the probe of the invention comprises a set of two or more labeled probes and the labeling groups to be detected are present each on a different probe, the labeling groups take such a spatial arrangement that the two chromophoric groups present on the two probes which have come to exist adjacent to each other only after the hybridization with the target nucleic acid induce unique phenomena such as excimer fluorescence, thereby enabling the target nucleic acid to be detected with a high recognizability. Thus the probe of the invention enables the erroneous recognition which has been problematic heretofore to be reduced remarkably and the types of utilizable labeling groups to be varied widely. Further it is possible to improve the detection sensitivity remarkably because it is possible to reduce the background noise remarkably. In addition, it is possible to discriminate a number of (single-base variation) nucleic acids that are different from one another in only one base present at a specific position to be detected, which has been difficult heretofore.
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Inventors:
Masayuki Masuko
Katsuyoshi Ebata
Katsuyoshi Ebata
Application Number:
JP52482296A
Publication Date:
October 17, 2007
Filing Date:
February 13, 1996
Export Citation:
Assignee:
Hamamatsu Photonics Co., Ltd.
International Classes:
C12Q1/68; C07H21/04; C12N15/09
Other References:
日本化学会講演予稿集, 1993年, 第65巻、第2号,第568頁, 1 H3 41
日本化学会講演予稿集, 1992年, 第63巻、第2号,第1894頁, 2 E743
日本化学会講演予稿集, 1992年, 第63巻、第2号, 第1894頁, 2 E744
電気化学および工業物理化学, 1998年, 第66巻, 第14-19頁
Photochem. Photobiol, 1995年, 第62巻, 第836-839頁
Nucl. Acids Symp. Ser., 1995年, 第34号, 第187-188頁
Nucl. Acids Res., 1998年, 第26巻, 第5409-5416頁
日本化学会講演予稿集, 1992年, 第63巻、第2号,第1894頁, 2 E743
日本化学会講演予稿集, 1992年, 第63巻、第2号, 第1894頁, 2 E744
電気化学および工業物理化学, 1998年, 第66巻, 第14-19頁
Photochem. Photobiol, 1995年, 第62巻, 第836-839頁
Nucl. Acids Symp. Ser., 1995年, 第34号, 第187-188頁
Nucl. Acids Res., 1998年, 第26巻, 第5409-5416頁
Attorney, Agent or Firm:
Yoshiki Hasegawa