Title:
植物ウイルスベクター
Document Type and Number:
Japanese Patent JP4009663
Kind Code:
B2
Abstract:
A novel plant virus vector and a method of gene expression into plants are provided, and the present invention relates to a plant virus vector characterized in that a part of the sequence of the 2b region of the RNA2 molecule of the Cucumber mosaic virus (CMV) is deleted; the aforementioned plant virus vector characterized in that the region from the StuI site to the stop codon of the 2b ORF of pCY2, which is an infectious clone of CMV-Y RNA2, is deleted; and a method of gene expression in plants characterized in that a foreign gene is introduced so as to be stably expressed in a plant using the genetic recombinant. This vector is useful as a plant RNA vector for causing stable expression of foreign genes.
Inventors:
Ken Matsumura
Masuda tax
Noriko Ichimachida
Masuda tax
Noriko Ichimachida
Application Number:
JP2003185981A
Publication Date:
November 21, 2007
Filing Date:
June 27, 2003
Export Citation:
Assignee:
National Institute of Advanced Industrial Science and Technology
Hokuren Agricultural Cooperative Federation
National University Corporation Hokkaido University
Hokuren Agricultural Cooperative Federation
National University Corporation Hokkaido University
International Classes:
C12N15/09; C12N5/10; C12N15/82; C12N15/83; A01H1/00; A01H5/00
Other References:
Virology,1991,183(1),p.106-13
Proc.Natl.Acad.Sci.U.S.A.,1996,93(15),p.7470-4
Mol.Plant Microbe Interact.,2002,15(9),p.947-55
EMBO J.,1995,14(23),p.5762-72
Proc.Natl.Acad.Sci.U.S.A.,1996,93(15),p.7470-4
Mol.Plant Microbe Interact.,2002,15(9),p.947-55
EMBO J.,1995,14(23),p.5762-72
Attorney, Agent or Firm:
Masahiko Sudo