Title:
ユーカリ属植物の成木を形質転換する方法
Document Type and Number:
Japanese Patent JP4099898
Kind Code:
B2
Abstract:
The present invention discloses a process for transforming mature trees of Eucalyptus plants comprising: inducing adventitious shoots from segments of the explant obtained from an adult tree of a Eucalyptus plant, preculturing the adventitious shoots in infection induction medium, infecting the adventitious shoots subjected to infection induction treatment with infection medium containing Agrobacterium tumefaciens, and rotary-culturing the infected explant segments in sterilization medium containing antibiotic; whereby sterilizing and forming transgenic calli, which regenerate transgenic plants by way of formation of shoot primordia by rotary-culturing under illumination.
Inventors:
Keigo Dohi
Keiko Kondo
Satoshi Kawazu
Keiko Kondo
Satoshi Kawazu
Application Number:
JP12702599A
Publication Date:
June 11, 2008
Filing Date:
May 07, 1999
Export Citation:
Assignee:
Oji Paper Co.,Ltd.
International Classes:
A01H1/00; C12N15/09; A01H4/00; C12N5/10; C12N15/82; C12N15/84
Domestic Patent References:
| JP7203790A | ||||
| JP10304785A | ||||
| JP8089113A |
Other References:
比留間直也 他,サクラ属・センダイヤの茎頂培養とAgrobacteriumを用いた遺伝子導入,育種学雑誌,日本,1994年 4月14日,第44巻、別冊1号,p.100.
Attorney, Agent or Firm:
Kanaya
Hiroshi Nakamoto
Akira Inoue
Hiroshi Nakamoto
Akira Inoue