Title:
ヒト誘導型一酸化窒素合成酵素の発現を制御する化合物のスクリーニング方法
Document Type and Number:
Japanese Patent JP4100712
Kind Code:
B2
Abstract:
A transformant prepared by constructing a plasmid wherein a human-inducible nitric oxide synthase (hiNOS) structural gene has been substituted by a luciferase structural gene which is a reporter gene while sustaining the functions of the 5'-promoter region and the 3'-untranslated region of the hiNOS gene and then stably trasferring the plasmid into an established human cell line. This transformant expresses selectively the reporter gene in the presence of an inducer. By determining the expression dose of the reporter gene owing to this transformant, it is possible to conveniently and sensitively screen compounds seemingly being useful in treating inflammation or sepsis through the inhibition of the expression of hiNOS. By inducing the expression thereof, further, it is possible to conveniently and sensitively screen compounds seemingly being useful as antitumor agents or antiviral agents or in treating re-angiostenosis inhibition.
Inventors:
Youichi Nunokawa
Oikawa Shinzo
Masaharu Tanaka
Oikawa Shinzo
Masaharu Tanaka
Application Number:
JP51451698A
Publication Date:
June 11, 2008
Filing Date:
September 18, 1997
Export Citation:
Assignee:
Asbiopharma Co., Ltd.
International Classes:
C12N15/09; A61K31/12; A61K31/165; A61K31/215; C07C50/06; C07C50/30; C07D295/185; C12N5/10; C12N9/02; C12Q1/02; C12Q1/25; C12Q1/68
Domestic Patent References:
JP62286949A |
Other References:
Biochem. Biophys. Res. Commun., 1996年 6月14日, vol.223, no.2, p.347-352
Attorney, Agent or Firm:
Hatsushi Shimizu
Kazunori Hashimoto
Kazunori Hashimoto