Title:
cp45HPIV―3株に基づいた弱毒化した生ワクチンおよびそのようなワクチンにおける弱毒化を確実にするための方法
Document Type and Number:
Japanese Patent JP4101878
Kind Code:
B2
Abstract:
The present invention is based upon the observation that the temperature sensitive phenotype of the cp45 strain of HPIV-3 correlates to a mutation in the large, or L, gene of cp45 relative to the corresponding gene in the wild-type strain. This correlation enables new vaccines directed at viruses other than HPIV-3 by combining, through genetic engineering methods, the region of the cp45 viral genome which encodes proteins responsible for replication and internal structure with the region of the genome of the target virus which encodes proteins responsible for attachment, penetration and release of the virus and virus progeny, respectfully. Moreover, it is possible to determine whether HPIV-3 or a cp45-hybrid virus is attenuated by confirming the presence or absence of mutations in its L gene.
Inventors:
Bersh, Robert Bee
Ray, Ranjit
Ray, Ranjit
Application Number:
JP52147997A
Publication Date:
June 18, 2008
Filing Date:
December 09, 1996
Export Citation:
Assignee:
ST.LOUIS UNIVERSITY
International Classes:
C12N7/04; C12N15/09; A61K31/00; A61K39/12; A61K39/155; A61P31/00; A61P31/12; A61P31/16; C07K14/115; C12N15/00; C12Q1/70; G01N33/15; A61K39/00
Other References:
1.J.Virology,Mar 1995,69(3)、1959-1963
2.J.Clin.Microbiol.30(8)p.2064-2070(1992)
3.J.Infect.Dis.172 p.1445-1450(1995)
4.Virus Res.30 p.43-52(1993)
5.J.Virol.70(1)p.580-584(1996)
2.J.Clin.Microbiol.30(8)p.2064-2070(1992)
3.J.Infect.Dis.172 p.1445-1450(1995)
4.Virus Res.30 p.43-52(1993)
5.J.Virol.70(1)p.580-584(1996)
Attorney, Agent or Firm:
Aoyama Aoi
Kyousei Tamura
Yushiro Tsuboi
Kyousei Tamura
Yushiro Tsuboi
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