Title:
アダプターに基づく配列分析の改良
Document Type and Number:
Japanese Patent JP4110216
Kind Code:
B2
Abstract:
An improvement in adaptor-based sequence analysis is provided that addresses the problems created by self-ligation of target polynucleotides that have complementary ends. The improvement includes preparation of target polynucleotides with dephosphorylated 5' strands and the use of adaptors having a 3' blocking group. In a preferred embodiment, adaptors are ligated to target polynucleotides by a single strand, 3' blocking groups are removed, the adjacent 5' hydroxyl of the target polynucleotide is phosphorylated, and the ligation of the adaptor is completed by treatment with a ligase.
Inventors:
Dubridge, Robert Bee.
Glenn Albrecht
Brenner, Sydney
Gryatsunov, Sergey M.
Macardi, Sarah N.
Glenn Albrecht
Brenner, Sydney
Gryatsunov, Sergey M.
Macardi, Sarah N.
Application Number:
JP54426098A
Publication Date:
July 02, 2008
Filing Date:
April 14, 1998
Export Citation:
Assignee:
Solexa,Inc.
International Classes:
C12N15/09; C12Q1/68; G01N33/543
Domestic Patent References:
| JP2000515006A |
Other References:
Nat. Biotechnol., vol. 18, pp. 630-634 (2000)
Proc. Natl. Acad. Sci. USA, vol. 97, pp. 1665-1670 (2000)
Proc. Natl. Acad. Sci. USA, vol. 97, pp. 1665-1670 (2000)
Attorney, Agent or Firm:
Yuichi Yamada
Masakazu Noda
Ikeda adult
Masakazu Noda
Ikeda adult