Title:
有核赤血球の定量のための血球溶解試薬組成物
Document Type and Number:
Japanese Patent JP4170903
Kind Code:
B2
Abstract:
A lytic reagent composition for measuring nucleated blood cells in a blood sample is described. The lytic reagent composition contains a quaternary ammonium surfactant, an ethoxylated phenol, and an ethoxylated alcohol. When mixed with a blood sample, the lytic reagent composition lyses red blood cells and enables a differentiation of nucleated red blood cells from other cell types by DC impedance measurement. The lytic reagent composition can further contain an organic ligand for determining total hemoglobin concentration of a blood sample photometrically. Further disclosed is a lytic reagent system including the lytic reagent composition and a diluent. In addition, a single reagent composition containing salts is also disclosed, which can be used without a separate diluent. The lytic reagent compositions can be used for concurrent measurement of nucleated red blood cells, WBC, and hemoglobin of a blood sample.
Inventors:
Lee
Lee, Jin
Lee, Jin
Application Number:
JP2003517569A
Publication Date:
October 22, 2008
Filing Date:
July 15, 2002
Export Citation:
Assignee:
Beckman Coulter, Incorporated
International Classes:
G01N33/72; C12Q1/02; G01N33/50; G01N33/80
Domestic Patent References:
| JP2000162209A | ||||
| JP2000502187A | ||||
| JP2000346839A | ||||
| JP2001509273A | ||||
| JP57013356A | ||||
| JP2000338104A | ||||
| JP2001524666A | ||||
| JP3252556A | ||||
| JP61088896A |
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Tetsuji Koga
Kazuhiro Nakamura
Masaya Nishiyama
Takashi Ishida
Tetsuji Koga
Kazuhiro Nakamura
Masaya Nishiyama