Title:
DNA重合の測定方法およびその方法の応用
Document Type and Number:
Japanese Patent JP4188228
Kind Code:
B2
Abstract:
A method for measuring DNA-dependent DNA polymerisation in a biological sample, is described. The method comprises the steps of providing a primer with a single stranded short specific sequence, which is unable to base pair internally, bound to a solid phase; contacting the primer construct with a reaction mixture containing a single stranded deoxynucleotide template with a part of the sequence complementary to the primer and the four deoxynucleoside triphosphates, one of which is modified so that it is specifically recognized by a labeled antibody; adding a biological sample comprising the DNA polymerase, such as retrovirus reverse transcriptase (RT), to the mixture; allowing the polymerase reaction to proceed; incubating the immobilized reaction product with the labeled antibody; detecting the amount of bound labeled antibody; and measuring the amount of incorporated modified deoxynucleoside triphosphate, as a measure of the DNA polymerisation, which may be used drug susceptibility testing. A commercial package is also disclosed.
Inventors:
Class, kelander
Ingbal, Paterson
Simon, Gronowitz
Shin Woo, Xiao
Ingbal, Paterson
Simon, Gronowitz
Shin Woo, Xiao
Application Number:
JP2003505360A
Publication Date:
November 26, 2008
Filing Date:
June 14, 2002
Export Citation:
Assignee:
CAVIDI TECH AB
International Classes:
C12N15/09; G01N33/50; C12Q1/68; C12Q1/6834; G01N33/15; G01N33/53; G01N33/566; C12Q1/70
Domestic Patent References:
| JP4148698A | ||||
| JP11504528A | ||||
| JP6509477A |
Foreign References:
| WO2001001129A1 |
Other References:
Biotechnol. Appl. Biochem.,1996年,Vol.23,pp.95-105
Attorney, Agent or Firm:
Kenji Yoshitake
Yukitaka Nakamura
Konno Akio
Noritaka Yokota
Yukitaka Nakamura
Konno Akio
Noritaka Yokota