Title:
逆転写酵素の高感度検出法
Document Type and Number:
Japanese Patent JP4256931
Kind Code:
B2
Abstract:
The present invention provides a method for detecting the presence of a retrovirus in a biological sample comprising the steps of: a) contacting the biological sample with an RNA template and a complementary DNA primer under conditions whereby the RNA template and the DNA primer will anneal and a DNA strand will be synthesized as an extension from the DNA primer if reverse transcriptase is present in the sample; b) amplifying the synthesized DNA; and c) detecting the amplification of the synthesized DNA, the amplification of the synthesized DNA indicating the presence of reverse transcriptase in the biological sample, thus indicating the presence of a retrovirus in the biological sample.
Inventors:
スウィッツァー，ウィリアム，マーシャル
フォルスク，トーマス，エム．
ヘネイン，ワリッド
ヤマモト，シンジ
フォルスク，トーマス，エム．
ヘネイン，ワリッド
ヤマモト，シンジ
Application Number:
JP52306396A
Publication Date:
April 22, 2009
Filing Date:
January 26, 1996
Export Citation:
Assignee:
アメリカ合衆国
International Classes:
C12N15/09; C12Q1/04; C12Q1/68; C12Q1/70; C12R1/92
Attorney, Agent or Firm:
川口 嘉之
松倉 秀実
遠山 勉
松倉 秀実
遠山 勉