Title:
ヌクレオシド-5’-燐酸エステルの製造法
Document Type and Number:
Japanese Patent JP4304727
Kind Code:
B2
Abstract:
A method for producing nucleoside-5'-phosphate ester involves phosphorylating nucleoside biochemically. Nucleoside-5'-phosphate ester is produced by allowing an acid phosphatase, especially an acid phosphatase having an increased affinity for a nucleoside and/or an increased temperature stability, to act at pH 3.0 to 5.5 on a nucleoside and a phosphate group donor. The phosphate group donor may be selected from polyphosphoric acid or a salt thereof, phenylphosphoric acid or a salt thereof, and carbamyl phosphate or a salt thereof. The resulting nucleoside-5'-phosphate ester may subsequently be collected. As an alternative to using isolated acid phosphatase to catalyse the reaction, a microorganism transformed with a gene encoding a protein having acid phosphatase activity may be employed.
Inventors:
Yasuhiro Mihara
Takashi Utagawa
Hideaki Yamada
Yasuhisa Asano
Takashi Utagawa
Hideaki Yamada
Yasuhisa Asano
Application Number:
JP16167497A
Publication Date:
July 29, 2009
Filing Date:
June 18, 1997
Export Citation:
Assignee:
AJINOMOTO CO.,LTD.
International Classes:
C12N15/09; C12N1/21; C12N9/16; C12N15/00; C12N15/55; C12P19/30; C12P19/36; C12R1/01; C12R1/185; C12R1/19; C12R1/22; C12R1/425
Domestic Patent References:
| JP9037785A | ||||
| JP7231793A |
Foreign References:
| WO1996037603A1 |
Other References:
Microbiology,1994年,Vol.140 No.6,p.1341-1350
International Journal of Systematic Bacteriology,1995年 4月,Vol.45 No.2,p.255-261
International Journal of Systematic Bacteriology,1995年 4月,Vol.45 No.2,p.255-261