Title:
不死化血管周皮細胞株
Document Type and Number:
Japanese Patent JP4321936
Kind Code:
B2
Abstract:
To provide an immortalized capillary pericyte line which maintains the original function/property of the cell line-deriving tissue, its establishment method, and the screening method for useful substance using the immortalized capillary pericyte line. Cerebral tissue of a transgenic rat carrying the large T antigen gene of SV40 thermo-sensitive mutant line tsA58 is homogenized and the resultant brain capillaries are treated with protease, thus obtained brain capillary cells are subcultured to establish an immortalized cell that expresses SV40 thermo-sensitive large T antigen, PDGF receptor beta , and Angiopoietin-1. In addition, the immortalized vascular pericyte line has ability to deposit calcium on matrix by dense culture.
Inventors:
Hisashi Izasa
Kenyuki Hattori
Nakajima Emi
Tetsuya Terasaki
Masuo Bando
Kenyuki Hattori
Nakajima Emi
Tetsuya Terasaki
Masuo Bando
Application Number:
JP2000037827A
Publication Date:
August 26, 2009
Filing Date:
February 16, 2000
Export Citation:
Assignee:
Japan Science and Technology Agency
International Classes:
A01K67/027; C07K14/025; C12N15/09; C12N5/071; C12N5/10; C12N15/18; C12N15/37; G01N33/15; G01N33/50
Domestic Patent References:
| JP11341982A |
Attorney, Agent or Firm:
Masaki Hirota