Title:
乳酸菌における異種遺伝子産物の製造のための改良発酵法
Document Type and Number:
Japanese Patent JP4399158
Kind Code:
B2
Abstract:
The invention provides a fed-batch or continuous process for producing heterologous peptides, polypeptides or proteins in lactic acid bacteria, comprising the steps of (i) constructing a recombinant lactic acid bacterium comprising a nucleotide sequence coding for the heterologous peptide, polypeptide or protein, and appropriate regulatory nucleotide sequences such as regulatable promoters and signal peptides to control the expression of the coding sequence and the secretion of gene product, (ii) cultivating the recombinant bacterium under or continuous cultivation conditions to express the gene, and (iii) harvesting the recombinant bacterium or the gene product. Preferably, the cultivation medium is a chemically defined or synthetic medium optionally supplemented with yeast extract.
Inventors:
Vrang, Astrid
Madsen, Shoren, Michelle
Bread Moss, Lars
Raven, Peter
Jose, Anau
Johnsen, Mads, Groenwald
Steenberg, Anne, Catherine
Hans, Israel Sen
Madsen, Shoren, Michelle
Bread Moss, Lars
Raven, Peter
Jose, Anau
Johnsen, Mads, Groenwald
Steenberg, Anne, Catherine
Hans, Israel Sen
Application Number:
JP2002536077A
Publication Date:
January 13, 2010
Filing Date:
October 19, 2001
Export Citation:
Assignee:
Bionier A/S
International Classes:
C12N15/09; C12N1/00; C12N1/20; C12N1/21; C12N9/16; C12N9/22; C12N15/67; C12N15/74; C12R1/01
Domestic Patent References:
| JP4075596A | ||||
| JP64016580A | ||||
| JP62138184A | ||||
| JP2174674A | ||||
| JP57202286A | ||||
| JP8500739A |
Foreign References:
| WO1998010079A1 |
Other References:
Gene,2000年 1月,Vol.242,p.347-56
Appl. Environ. Microbiol.,1993年,Vol.59, No.12,p.4363-6
Appl. Environ. Microbiol.,1993年,Vol.59, No.12,p.4363-6
Attorney, Agent or Firm:
Shintaro Nogawa