Title:
組み込まれたシグナリング系に使用するためのPCRプライマー・コンストラクト
Document Type and Number:
Japanese Patent JP4440469
Kind Code:
B2
Abstract:
A method for the detection of a target nucleic acid, which method comprises contacting template nucleic acid from a sample with (i) a signalling system and (ii) a tailed nucleic acid primer having a template binding region and the tail comprising a linker and a target binding region, in the presence of appropriate nucleoside triphosphates and an agent for polymerisation thereof, under conditions such that the template binding region of the primer will hybridise to a complementary sequence in the template nucleic acid and be extended to form a primer extension product, separating any such product from the template whereupon the target binding region in the tail of the primer will hybridise to a sequence in the primer extension product corresponding to the target nucleic acid, and wherein any such target specific hybridisation causes a detectable change in the signalling system, such that the presence or absence of the target nucleic acid in the sample is detected by reference to the presence or absence of a detectable change in the signalling system.
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Inventors:
Gibson, Neil James
Little, steven
Sheakar, Jane
White Coomb, David Mark
Little, steven
Sheakar, Jane
White Coomb, David Mark
Application Number:
JP2000554879A
Publication Date:
March 24, 2010
Filing Date:
November 25, 1998
Export Citation:
Assignee:
Dxs Limited
International Classes:
C12Q1/68; C12N15/09; G01N33/50; C12Q1/6818; C12Q1/6823; C12Q1/6853; G01N33/58
Domestic Patent References:
| JP6007199A |
Other References:
Human Mutation,1998, Vol.11, pp.252-258
Attorney, Agent or Firm:
Kazuo Shamoto
Tadashi Masui
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Hiroko Ejiri
Tadashi Masui
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Hiroko Ejiri