Title:
ヒトパピローマウイルスのウイルス様粒子の精製方法
Document Type and Number:
Japanese Patent JP4440471
Kind Code:
B2
Abstract:
A process for purifying papillomavirus virus-like particles (VLPs) includes the step of passing a partially purified VLP-containing solution through a hydroxyapatite chromatography column. The VLPs are then eluted using a buffer containing phosphate anion. The advantages of this method include the recovery of a high yield of intact VLPs.
Inventors:
Kutsuku, the aims sea, the third
Application Number:
JP2000565108A
Publication Date:
March 24, 2010
Filing Date:
August 10, 1999
Export Citation:
Assignee:
MERCK & COMPANY INCOPORATED
International Classes:
C07K14/025; C12N15/09; A61K39/12; A61P35/00; C07K1/16; C12N7/02; C12N7/04; C12P21/00; C12R1/865
Domestic Patent References:
| JP10500847A |
Foreign References:
| WO1997008298A1 |
Other References:
Bio-Medical Materials and Engineering, 1991, Vol.1, p.143-147
Attorney, Agent or Firm:
Yoshio Kawaguchi
Naoya Fushimi
Makoto Ono
Naoya Fushimi
Makoto Ono