Title:
部分的に乾燥した1つ以上の酵素を含む安定化された混合物の製造方法、混合物、および混合物を含有するキット
Document Type and Number:
Japanese Patent JP4448280
Kind Code:
B2
Abstract:
The invention relates to a method consisting of bringing into contact, in one container, (a) an aqueous solution of a reaction mixture comprising at least one enzyme, and (b) an aqueous solution of a stabilizing mixture comprising (i) at least one protective agent against drying, (ii) at least one inhibitor of the condensation reaction between carbonyl or carboxyl groups and amine or phosphate groups, and (iii) at least one inert polymer capable of generating a mesh structure preventing the mobility of the dried reagents. The invention also consists of removing all or part of the water contained in the resulting aqueous solution. Said process is suitable for carrying out enzymatic reactions, for example, amplifying, sequencing and characterizing nucleic acids, performing hybridization tests and for the restriction analysis.
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Inventors:
Manuel Franco de Saravia Rosado, Pedro
Gemma Limmons Lopez
Antonio Madejon Cayce
Maria, Dolores Marine Alvardi
Gemma Limmons Lopez
Antonio Madejon Cayce
Maria, Dolores Marine Alvardi
Application Number:
JP2002570962A
Publication Date:
April 07, 2010
Filing Date:
March 11, 2002
Export Citation:
Assignee:
Biotors Biotechnology and Medical Laboratories, S.A.
International Classes:
C12N9/16; C12N9/12; C12N9/96; C12Q1/68
Domestic Patent References:
| JP10503383A | ||||
| JP10505591A | ||||
| JP2000513940A | ||||
| JP10511858A | ||||
| JP2000514298A | ||||
| JP8131171A |
Foreign References:
| WO1993000807A1 | ||||
| US6300073 |
Attorney, Agent or Firm:
Makoto Hagiwara