Title:
移動度改変ヌクレオ塩基ポリマーおよび同一物を使用する方法
Document Type and Number:
Japanese Patent JP4452445
Kind Code:
B2
Abstract:
The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.
Inventors:
Woo, Samuel.
Graham, Ron
Tian, Gin
Graham, Ron
Tian, Gin
Application Number:
JP2002581694A
Publication Date:
April 21, 2010
Filing Date:
April 15, 2002
Export Citation:
Assignee:
Applied Biosystems, LLC
International Classes:
C07H21/00; G01N33/53; C08G65/335; C12N15/09; C12Q1/68; G01N27/447; G01N37/00
Domestic Patent References:
| JP7505529A | ||||
| JP8504082A |
Foreign References:
| US3172871 |
Other References:
HERDEWIJN, P., et al.,Helv. Chim. Acta,72,pp.1739-1748 (1989)
KUIJPERS, W.H.A., et al.,Tetrahedron,49(47),pp.10931-10944 (1993)
SEELIG, B., et al.,Tetrahedron Lett.,38(44),pp.7729-7732 (1997)
CONNOLLY, B.A.,Tetrahedron Lett.,28(4),pp.463-466 (1987)
MIZRAKH, L.I., et al.,J. Gen. Chem. USSR,45(10),p.2301
VERHEIJEN, J.C., et al.,Bioorg. Med. Chem.,7,pp.449-455 (1999)
RUMNEY, IV, S., et al.,J. Am. Chem. Soc.,117,pp.5635-5646 (1995)
KUIJPERS, W.H.A., et al.,Tetrahedron,49(47),pp.10931-10944 (1993)
SEELIG, B., et al.,Tetrahedron Lett.,38(44),pp.7729-7732 (1997)
CONNOLLY, B.A.,Tetrahedron Lett.,28(4),pp.463-466 (1987)
MIZRAKH, L.I., et al.,J. Gen. Chem. USSR,45(10),p.2301
VERHEIJEN, J.C., et al.,Bioorg. Med. Chem.,7,pp.449-455 (1999)
RUMNEY, IV, S., et al.,J. Am. Chem. Soc.,117,pp.5635-5646 (1995)
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita