Title:
アグロバクテリウム・ラジオバクターP230由来のホスホトリエステラーゼ
Document Type and Number:
Japanese Patent JP4454937
Kind Code:
B2
Abstract:
The present invention provides enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention provides a phosphotriesterase enzyme identified from an Agrobacterium radiobacter strain isolated from soil that hydrolyses OP pesticides, and the gene encoding that enzyme. The invention also provides mutants of the identified phosphotriesterase enzyme which have altered substrate specificity. The use of these enzymes in bioremediation strategies is also provided.
Inventors:
Irene Horne
Tara Chatterland
Rebecca Harcourt
Robin Russell
John Oakshot
Tara Chatterland
Rebecca Harcourt
Robin Russell
John Oakshot
Application Number:
JP2002589671A
Publication Date:
April 21, 2010
Filing Date:
May 15, 2002
Export Citation:
Assignee:
COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION
International Classes:
A01H5/00; C12N15/09; B09C1/02; B09C1/08; C02F1/00; C12N1/15; C12N1/19; C12N1/20; C12N1/21; C12N5/10; C12N9/16; C12Q1/00; C12Q1/02
Domestic Patent References:
| JP3501086A |
Foreign References:
| WO2000065081A1 |
Other References:
J. Biol. Chem,1989年,vol.264,p.19659-19665
J. Bacteriol.,1989年,vol.171,p.6740-6746
J. Bacteriol.,1989年,vol.171,p.6740-6746
Attorney, Agent or Firm:
Yasuhiko Murayama
Masatake Shiga
Takashi Watanabe
Shinya Mitsuhiro
Masatake Shiga
Takashi Watanabe
Shinya Mitsuhiro