Title:
脊椎動物における遺伝子移入のためのトランスポゾンベクターであるFrogPrince
Document Type and Number:
Japanese Patent JP4493492
Kind Code:
B2
Abstract:
The present invention relates to a transposon-based DNA integration system comprising (a) a transposon which is devoid of a polynucleotide encoding a functional transposase and which comprises a polynucleotide of interest, wherein the transposon comprises inverted repeats having a degree of identity with the repeats within SEQ ID NO: 2 and its inverted repeat, respectively, of at least 90%; and (b) a transposase having at its N-terminus a DNA binding domain comprising the sequences of SEQ ID NO: 3 and 4; or (c) a polynucleotide encoding the transposase of (b). The present invention further relates to a method of transferring a polynucleotide of interest into cells of a vertebrate comprising the step of introducing the transposon-based DNA integration system of the invention into said cells. In addition, the invention relates to a method of effecting RNAi comprising (a) stably introducing a transposon comprising an expression cassette expressing a short interfering RNA and a selectable marker gene as part of the transposon-based DNA integration system of the invention into a cell; (b) selecting for cells expressing the selectable marker; and (c) assessing whether the transcription/translation of the desired gene is effected by RNAi. A further embodiment of the invention is a method of gene trapping genes comprising the steps of (a) introducing the transposon-based DNA integration system of the invention into a cell; and (b) assessing for the expression of a selectable marker wherein expression of a selectable marker is indicative of integration of the transposon into a transcibed gene of the cell.
Inventors:
Miscay, Chava
Ijuvac, Jujanna
Ivic, Zoltan
Plasterk, ronalto
Ijuvac, Jujanna
Ivic, Zoltan
Plasterk, ronalto
Application Number:
JP2004507510A
Publication Date:
June 30, 2010
Filing Date:
May 30, 2003
Export Citation:
Assignee:
Max-Delbrück-Zentrum Fure Moleculare Medicin
Netherlands Cancer Institute
Netherlands Cancer Institute
International Classes:
A01K67/027; C12N15/09; A61K48/00; C12N5/10; C12N15/85; C12N15/90
Other References:
Nucleic Acids Res.(2003 Dec),Vol.31,No.23,p.6873-6881
Cell(1997),Vol.91,p.501-510
Cell(1997),Vol.91,p.501-510
Attorney, Agent or Firm:
Kenzo Hara International Patent Office
Kenzo Hara
Ryuichi Kijima
Ichiro Kaneko
Kenzo Hara
Ryuichi Kijima
Ichiro Kaneko