Title:
封入体結合タンパク質をコードする遺伝子(ibpAおよび/またはibpB)を欠失あるいは増幅させて目的タンパク質を製造する方法
Document Type and Number:
Japanese Patent JP4531570
Kind Code:
B2
Abstract:
A method for producing target proteins by deleting or amplifying ibpA and/or ibpB genes coding for inclusion body-associated proteins. Two methods for producing target proteins using ibpA and/or ibpB genes coding for inclusion body-associated proteins of E. coli are described. The first method enhances the secretory production and activity of target proteins using ibpA and/or ibpB genes-deleted bacteria. The second method enhances the production of target proteins in the cytoplasm and also converts the target proteins from soluble form to insoluble inclusion body, using ibpA and/or ibpB gene-amplified bacteria.
More Like This:
Inventors:
Issanyup
Han Mee Jun
Pakshiye
Han Mee Jun
Pakshiye
Application Number:
JP2004569376A
Publication Date:
August 25, 2010
Filing Date:
July 10, 2003
Export Citation:
Assignee:
ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTE
International Classes:
C12N15/09; C07K14/245; C07K14/54; C07K14/57; C07K14/65; C12N1/21; C12N9/16; C12N15/70; C12P21/02
Other References:
FEBS Lett.,2003年 2月27日,Vol.537,p.215-221
Mol. Microbiol.,2000年,Vol.36, No.6,p.1360-1370
FEMS Microbiol. Lett.,2000年,Vol.184,p.165-171
Gene,1993年,Vol.134,p.1-6
J. Biotechnol.,2002年,Vol.96,p.3-12
Microbiology,2002年,Vol.148,p.1757-1765
Eur. J. Biochem.,2002年,Vol.269,p.2907-2917
J. Bacteriol.,1998年,Vol.180, No.19,p.5165-5172
Acta Biochim. Pol.,2003年,Vol.50, No.1,p.239-247
Mol. Microbiol.,2000年,Vol.36, No.6,p.1360-1370
FEMS Microbiol. Lett.,2000年,Vol.184,p.165-171
Gene,1993年,Vol.134,p.1-6
J. Biotechnol.,2002年,Vol.96,p.3-12
Microbiology,2002年,Vol.148,p.1757-1765
Eur. J. Biochem.,2002年,Vol.269,p.2907-2917
J. Bacteriol.,1998年,Vol.180, No.19,p.5165-5172
Acta Biochim. Pol.,2003年,Vol.50, No.1,p.239-247
Attorney, Agent or Firm:
Kenichi Morita
Kenjiro Yamaguchi
Kenjiro Yamaguchi