Title:
糖タンパク質のための細胞培養方法
Document Type and Number:
Japanese Patent JP4543131
Kind Code:
B2
Abstract:
A glycoprotein is produced by a process comprising culturing mammalian host cells expressing nucleic acid encoding said glycoprotein in the presence of (a) a factor that modifies growth state in a cell culture, (b) a divalent metal cation that can adopt and prefers an octahedral coordination geometry, and/or (c) a plasma component. In this process, the occupancy of an N-linked glycosylation site occupied only in a fraction of a glycoprotein is enhanced. Such culturing is preferably carried out at a temperature of between about 30° C. and 35° C. and/or in the presence of up to about 2 mM of a butyrate salt and/or in the presence of a cell-cycle inhibitor.
Inventors:
Andersen, Dana, See.
Bridgez, Tiffany, M.
Gauricek, Martin
Hui, Cynthia, A.
Bridgez, Tiffany, M.
Gauricek, Martin
Hui, Cynthia, A.
Application Number:
JP2000614404A
Publication Date:
September 15, 2010
Filing Date:
April 21, 2000
Export Citation:
Assignee:
Genentech, Incorporated
International Classes:
C12N9/64; C12N5/10; C12N9/72; C12N15/58; C12P21/00; C12P21/04; C12R1/91
Other References:
OLIVEIRA C M,JOURNAL OF CELLULAR PHYSIOLOGY,1990年,V144 N3,P467-472
HOSOI SINJI,SYTOTECHNOLOGY,1996年,V19 N2,P125-135
Fibrinolysis and Proteolysis,1998, Vol.12 ,No.1, pp.17-22
Cytotechnology,1999, Vol.29, pp.55-64
Fibrinolysis,1995, Vol.9, pp.293-297
Biochemistry,1995, Vol.34, pp.9444-9450
Cytotechnology,1995, Vol.17, pp.13-19
Experimental Cell Research,1988, Vol.175, pp.26-36
Biotechnology and Bioengineering,1997, Vol.55, No.2, pp.328-338
HOSOI SINJI,SYTOTECHNOLOGY,1996年,V19 N2,P125-135
Fibrinolysis and Proteolysis,1998, Vol.12 ,No.1, pp.17-22
Cytotechnology,1999, Vol.29, pp.55-64
Fibrinolysis,1995, Vol.9, pp.293-297
Biochemistry,1995, Vol.34, pp.9444-9450
Cytotechnology,1995, Vol.17, pp.13-19
Experimental Cell Research,1988, Vol.175, pp.26-36
Biotechnology and Bioengineering,1997, Vol.55, No.2, pp.328-338
Attorney, Agent or Firm:
Sonoda Yoshitaka
Kobayashi Yoshinori
Kobayashi Yoshinori