Title:
環状ヌクレオチドの細胞内濃度を決定する試験方法
Document Type and Number:
Japanese Patent JP4566984
Kind Code:
B2
Abstract:
The invention relates to methods for the quantitative optical analysis of the intracellular concentration of the cyclic nucleotides cGMP and cAMP, said methods using cell lines which express a combination of certain CNG channels, a calcium-sensitive photoprotein, and different target proteins for which modulators are to be found, in a recombinant manner. The cell lines modified in this way are suitable for high-throughput screening (HTS and uHTS) and can be used to identify medicaments which influence the activity of receptors or enzymes participating in the composition or decomposition of the cyclic nucleotides cGMP and cAMP.
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Inventors:
Frank Wunder
Application Number:
JP2006504569A
Publication Date:
October 20, 2010
Filing Date:
March 06, 2004
Export Citation:
Assignee:
AKZAM Societa Peratini
International Classes:
C12Q1/68; C12N5/10; C12N15/09; C12Q1/02; C12Q1/26; C12Q1/44; C12Q1/527; G01N33/542; G01N33/573; G01N33/68
Foreign References:
| WO2003014149A1 |
Other References:
Proc Natl Acad Sci USA, (1991), vol.88, p.9868-9872
Proc Natl Acad Sci USA, (1998), vol.95, p.4696-4701
Proc Natl Acad Sci USA, (1998), vol.95, p.4696-4701
Attorney, Agent or Firm:
Mitsuo Tanaka
Hiroshi Yamazaki
Michiko Matsutani
Mina Shiga
Sakurai Yoko
Satoshi Hashimoto
Hiroshi Yamazaki
Michiko Matsutani
Mina Shiga
Sakurai Yoko
Satoshi Hashimoto