Title:
多糖類をタンパクに結合する方法
Document Type and Number:
Japanese Patent JP4619459
Kind Code:
B2
Abstract:
A novel method of covalently coupling polysaccharides to other biopolymers is provided, using an amino-thiol linker represented by formula 1 H2N-Ä(CH2)m-CHR<1>-CR<2>R<3>-AÜq-CHR<4>-(CHR<5>)p-CHR<6>-S-R<7> wherein A is a direct bond or a group having the formula -äZ-(CH2)m-CHR<1>-CHR<2>R<3>ünZ-, m is an integer from 0 to 5; n is an integer from 0 to 3; p is an integer from 0 to 2; q is the integer 0 or 1; R<1> is hydrogen or C1-C6 alkyl, optionally substituted by amino, hydroxyl, carboxyl, C1-C4 alkoxycarbonyl, carbamoyl, mono- or di-C1-C4-alkylcarbamoyl or N-( alpha -carboxyalkyl)carbamoyl, or, if m NOTEQUAL 0, R<1> is hydroxyl, amino or peptidyl-amino; R<2> and R<3> are independently hydrogen or C1-C4 alkyl, or together form an oxo group; R<4> is hydrogen, C1-C4 alkyl, carboxyl, C1-C4 alkoxycarbonyl, carbamoyl, mono- or di-C1-C4-alkylcarbamoyl or N-( alpha -carboxyalkyl)carbamoyl; R<5> is hydrogen, methyl, hydroxy or C1-C7 acyloxy; R<6> is hydrogen or methyl; R<7> is hydrogen or a thiol-protecting group or a group having the formula -S-CHR<6>-(CHR<5>)p-CHR<4>-ÄA-CR<2>R<3>-CHR<1>-(CH2)mÜq-NH2; and Z is imino, methylimino, oxygen or sulphur. ,
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Inventors:
Hogeroth, peter
Application Number:
JP52756498A
Publication Date:
January 26, 2011
Filing Date:
December 16, 1997
Export Citation:
Assignee:
De Stadt der Naderlanden, Verteggen Uhldikt D'or de Minister van Berzin, Volksguessonde Hyde en Karthur
International Classes:
A61K39/00; A61K39/09; C08B37/00; A61K47/48; C07K1/107; C07K9/00
Foreign References:
| EP0326111A1 |
Attorney, Agent or Firm:
Sadao Muramatsu
Ryo Hashimoto
Toshio Shirane
Ryo Hashimoto
Toshio Shirane