Title:
感染性クローン
Document Type and Number:
Japanese Patent JP4637432
Kind Code:
B2
Abstract:
The present invention relates to methods of preparing a DNA comprising steps, wherein (a) a DNA comprising a full length copy of the genomic RNA (gRNA) of an RNA virus; or (b) a DNA comprising one or several fragments of a gRNA of an RNA virus, which fragments code for an RNA dependent RNA polymerase and at least one structural or non-structural protein; or (c) a DNA having a homology of at least 60% to the sequences of (a) or (b); is cloned into a bacterial artificial chromosome (BAC). Additionally, DNAs are provided, which comprise sequences derived from the genomic RNA (gRNA) of a coronavirus which sequences have a homology of at least 60% to the natural sequence of the virus and code for an RNA dependent RNA polymerase and at least one structural or non-structural protein, wherein a fragment of said DNA is capable of being transcribed into RNA which RNA can be assembled to a virion. Further, the use of these nucleic acids for preparation of viral RNA or virions as well as pharmaceutical preparations comprising these DNAs, viral RNAs or virions is disclosed.
Inventors:
En Juanes Sanchez, Lewis
Application Number:
JP2001541529A
Publication Date:
February 23, 2011
Filing Date:
November 30, 2000
Export Citation:
Assignee:
CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
International Classes:
C12N15/09; A61K35/74; A61K35/76; A61K39/00; A61K39/225; A61K39/395; A61K48/00; A61P1/00; A61P11/00; A61P29/00; A61P31/00; A61P31/12; C07K14/08; C12N1/21; C12N7/00; C12N7/04; C12N15/50; C12N15/70; C12N15/86; C12R1/19
Foreign References:
| WO1999043842A1 |
Other References:
Luis Enjuanesら,Coronaviruses and arteriviruses,Plemium Press・New York and London,1998年,265-268
Luis Enjuanesら,Coronaviruses and arteriviruses,Plemium Press・New York and London,,1998年,319-325
Proc Natl Acad Sci USA, vol.89, p.8794-8797 (1992)
Proc Natl Acad Sci USA, vol.97, p.5516-5521 (2000)
Luis Enjuanesら,Coronaviruses and arteriviruses,Plemium Press・New York and London,,1998年,319-325
Proc Natl Acad Sci USA, vol.89, p.8794-8797 (1992)
Proc Natl Acad Sci USA, vol.97, p.5516-5521 (2000)
Attorney, Agent or Firm:
Kato Asamichi
Kiuchi Uchida
Yasumasa Ishida
Kiuchi Uchida
Yasumasa Ishida