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Title:
フローサイトメータ、細胞の解析方法、細胞解析プログラム、蛍光検出器の感度設定方法および陽性率判定法における基準ゲート設定方法
Document Type and Number:
Japanese Patent JP4649231
Kind Code:
B2
Abstract:

To provide a flow site meter which can judge living cells and died cells without a fluorescent light detector and a cell viability test reagent.

This flow site meter 1 is characterized by comprising a correlation diagram-making means 8 for making a correlation diagram from a first scattered light-measured data and a second scattered light-measured data, a difference information calculation means 9 for calculating difference information between the correlation diagrams, a threshold-comparing means 15 for comparing the difference information with a threshold to extract a largely increased region and a largely decreased region from the difference information, a boundary line for dividing the largely increased region and the largely decreased region, a boundary line-superimposing treatment means 17 for superimposing the boundary line on the correlation diagram to divide the correlation diagram, thus setting living and died cell regions in the correlation diagram, and a measured data-extracting means 18 for extracting the measured data of cell contained in the living and died cell regions.

COPYRIGHT: (C)2006,JPO&NCIPI


Inventors:
Ozawa Osamu
Application Number:
JP2005052465A
Publication Date:
March 09, 2011
Filing Date:
February 28, 2005
Export Citation:
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Assignee:
Kaneka Corporation
International Classes:
C12M1/00; G01N15/14; C12N15/09; G01N21/78; G01N33/53; G01N33/536
Domestic Patent References:
JP2003304867A
JP5209821A
JP8271509A
JP2500297A
Foreign References:
WO2006051813A1
Attorney, Agent or Firm:
Isono Dozo