Title:
キャピラリー電気泳動法によるヘモグロビンの分析方法およびそれに用いる添加剤
Document Type and Number:
Japanese Patent JP4660653
Kind Code:
B2
Abstract:
The present invention provides a method for analyzing hemoglobin by capillary electrophoresis, that allows the apparatus to be smaller in size, allows a highly precise analysis to be obtained, and allows the analysis to be performed in a short period of time. The analytical method of the present invention are methods for analyzing hemoglobin by capillary electrophoresis, comprising: a sample-providing step of providing a sample containing hemoglobin; a capillary tube-providing step of providing a capillary tube containing a buffer solution; and an electrophoresis step of carrying out electrophoresis of the sample, by introducing the sample into the buffer solution in the capillary tube, and applying a voltage across both ends of the capillary tube; wherein the electrophoresis is carried out following at least one of modes (A) and (B) below: (A) the electrophoresis is carried out with a surfactant (a) added to the buffer solution, the surfactant (a) being a non-ionic surfactant having an alkyl group as a hydrophobic portion and a sugar as a hydrophilic portion; and (B) the electrophoresis is carried out with a surfactant (b) added to the sample, the surfactant (b) being a betaine-type amphoteric surfactant.
Inventors:
Yusuke Nakayama
Yonehara Satoshi
Yonehara Satoshi
Application Number:
JP2008535811A
Publication Date:
March 30, 2011
Filing Date:
April 23, 2008
Export Citation:
Assignee:
Arkray Co., Ltd.
International Classes:
G01N27/447; G01N33/72
Domestic Patent References:
| JP2004077457A | 2004-03-11 | |||
| JP2006145537A | 2006-06-08 | |||
| JPH09105739A | 1997-04-22 | |||
| JPH11295286A | 1999-10-29 | |||
| JPH11223624A | 1999-08-17 | |||
| JP2003294752A | 2003-10-15 | |||
| JP2008256460A | 2008-10-23 | |||
| JP2008267819A | 2008-11-06 |
Attorney, Agent or Firm:
Koichiro Tsujimaru
Yumi Nakayama
Reiko Yoshida
Isaji So
Yumi Nakayama
Reiko Yoshida
Isaji So