Title:
生物分子の生成方法及び装置
Document Type and Number:
Japanese Patent JP4699357
Kind Code:
B2
Abstract:
A scalable process and device for producing a biomolecule, in particular pharmaceutical grade plasmid DNA. The process includes the steps of alkaline lysis and a neutralization. For separating the lysate and the precipitate, the mixture is allowed to gently flow downward through a clarification reactor that is partially filled, in its lower part, with retention material like glass beads, whereby the precipitate is retained on top of and within the retention. In a preferred embodiment of the lysis step, cell suspension and alkaline lysis solution flow through a lysis reactor that is filled with particulate material like glass beads. The process can be run continuosly and fully automated.
Inventors:
Ultara Yochen
Nesina roman
Asher Christine
Velor Helga
Nesina roman
Asher Christine
Velor Helga
Application Number:
JP2006504817A
Publication Date:
June 08, 2011
Filing Date:
March 23, 2004
Export Citation:
Assignee:
Boehringer Ingelheim Elzefau Gesellschaft Mitt Beschlenktel Haftung Und Company Comandito Gesellschaft
International Classes:
C12N15/09; C12M1/00; C12N1/06; C12N15/10
Domestic Patent References:
| JP5505848A | ||||
| JP2002262855A | ||||
| JP7501223A | ||||
| JP7250681A | ||||
| JP2002345465A | ||||
| JP2003527821A | ||||
| JP2001095572A |
Foreign References:
| WO2000009680A1 |
Attorney, Agent or Firm:
Sadao Kumakura
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Koji Hirayama
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Koji Hirayama