Title:
タンパク質の固定化方法
Document Type and Number:
Japanese Patent JP4754352
Kind Code:
B2
Abstract:
This invention provides a method for immobilizing proteins comprising: step 1 of purifying target proteins to be immobilized, which have a first tag portion and a second tag portion, with the use of the first tag portion; step 2 of activating reactive groups capable of covalently binding to the proteins on a carrier for immobilization; and step 3 of allowing a solution containing the proteins purified in step 1 to react with the carrier after step 2, wherein, in step 3, the proteins are immobilized on the carrier via interactions between the second tag portion and the site of the carrier to which the second tag portion binds and via covalent binding between the reactive groups and the proteins. This method enables the stable immobilization of various types of target proteins on a carrier regardless of the amounts of target proteins and without nonspecific immobilization of contaminating proteins.
Inventors:
Go Yamada
Ryomasa Suwa
Tsutsumi Tsuyoshi
Ryomasa Suwa
Tsutsumi Tsuyoshi
Application Number:
JP2005513496A
Publication Date:
August 24, 2011
Filing Date:
August 24, 2004
Export Citation:
Assignee:
Reverse Proteomics Laboratory Inc.
International Classes:
G01N33/543; G01N37/00
Domestic Patent References:
| JP2001505764A | 2001-05-08 | |||
| JPH10512140A | 1998-11-24 | |||
| JP2002523059A | 2002-07-30 | |||
| JPH11512518A | 1999-10-26 | |||
| JP2002520621A | 2002-07-09 |
Foreign References:
| WO2001072458A1 | 2001-10-04 | |||
| WO2002033044A2 | 2002-04-25 | |||
| WO2003056336A2 | 2003-07-10 |
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Satoshi Fujita
Ishii Teiji
Satoshi Fujita